synthetic ns1 proteins  (Native Antigen Inc)

Journal: Viruses

Article Title: NS1 Antigenemia and Viraemia Load: Potential Markers of Progression to Dengue Fatal Outcome?

doi: 10.3390/v10060326

Figure Lengend Snippet: Non-structural protein 1 (NS1) antigenemia ( A ) according to the disease outcome (non-fatal: n = 40; fatal: n = 40), * p < 0.01 using t test; ( B ) in the overall analysis of the type of infection (primary: n = 24 versus secondary: n = 45) using t test; ( C ) in the analysis of primary non-fatal ( n = 8) versus fatal ( n = 16) cases by t test; ( D ) in the secondary non-fatal ( n = 23) and fatal ( n = 22) cases, * p < 0.01 using t test; ( E ) in the analysis of DENV serotype regardless of the disease outcome ( n = 80) by ANOVA test * p < 0.01; ( F ) in the analysis of the distinct DENV serotypes according to the disease outcome (fatal: n = 10 and non-fatal/serotype) using t test. For DENV-2, fatal versus non-fatal cases, * p < 0.01, and for DENV-4 fatal versus non-fatal cases, * p < 0.01.

Article Snippet: Briefly, a standard NS1 antigen curve (ng/mL) based on an equation (y = 1.321x + 0.1271) with R 2 = 0.9542 was used and established using synthetic NS1 proteins (Native Antigen Company, Oxforshire, UK) corresponding to the NS1 of DENV-1 (Nauru/Western Pacific/1974), DENV-2 (Thailand/16681/84), DENV-3 (Sri Lanka D3/H/IMTSSA-SRI/2000/1266) and DENV-4 (Dominica/814669/1981) with 10-fold dilution.

Techniques: Infection

Journal: Viruses

Article Title: NS1 Antigenemia and Viraemia Load: Potential Markers of Progression to Dengue Fatal Outcome?

doi: 10.3390/v10060326

Figure Lengend Snippet: Overall NS1 antigenemia and RNA viral load according to the distinct DENV serotypes, independent of the disease outcome.

Article Snippet: Briefly, a standard NS1 antigen curve (ng/mL) based on an equation (y = 1.321x + 0.1271) with R 2 = 0.9542 was used and established using synthetic NS1 proteins (Native Antigen Company, Oxforshire, UK) corresponding to the NS1 of DENV-1 (Nauru/Western Pacific/1974), DENV-2 (Thailand/16681/84), DENV-3 (Sri Lanka D3/H/IMTSSA-SRI/2000/1266) and DENV-4 (Dominica/814669/1981) with 10-fold dilution.

Techniques:

Journal: Viruses

Article Title: NS1 Antigenemia and Viraemia Load: Potential Markers of Progression to Dengue Fatal Outcome?

doi: 10.3390/v10060326

Figure Lengend Snippet: NS1 antigenemia and RNA viral load according to the distinct DENV serotype and disease outcome.

Article Snippet: Briefly, a standard NS1 antigen curve (ng/mL) based on an equation (y = 1.321x + 0.1271) with R 2 = 0.9542 was used and established using synthetic NS1 proteins (Native Antigen Company, Oxforshire, UK) corresponding to the NS1 of DENV-1 (Nauru/Western Pacific/1974), DENV-2 (Thailand/16681/84), DENV-3 (Sri Lanka D3/H/IMTSSA-SRI/2000/1266) and DENV-4 (Dominica/814669/1981) with 10-fold dilution.

Techniques:

Journal: Interdisciplinary Perspectives on Infectious Diseases

Article Title: The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

doi: 10.1155/2022/1639990

Figure Lengend Snippet: Primers used for PCR analysis.

Article Snippet: The full length of a synthetic gene encoding hPVB19 NS1 protein (NCBI Reference Sequence: NC_000883.2) was received in the pCMV6-AC-GFP-NS1 constitutive expression vector (Biomatik, Ontario, Canada).

Techniques: Sequencing

Journal: Interdisciplinary Perspectives on Infectious Diseases

Article Title: The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

doi: 10.1155/2022/1639990

Figure Lengend Snippet: (a) Expression of GFP in NS1-transfected HEK-293T cells was examined by fluorescence microscopy at (I) 24 hr (II) 48 hr (III) 72 hr posttransfection with 10x magnification and bar size of 25 μ m. (b) The expression of NS1 mRNA in HEK-293 T cells was examined and visualized on 1.5% agarose gel at 100 V for 40 min and stained with GelRed at the indicated times posttransfection. (c) Posttransfection expression levels of NS1 mRNA overtime. Green: after 24 hr, red: after 48 hr, blue: after 72 hr, brown: mock-transfection at 24, 48, and 72 hr posttransfection.

Article Snippet: The full length of a synthetic gene encoding hPVB19 NS1 protein (NCBI Reference Sequence: NC_000883.2) was received in the pCMV6-AC-GFP-NS1 constitutive expression vector (Biomatik, Ontario, Canada).

Techniques: Expressing, Transfection, Fluorescence, Microscopy, Agarose Gel Electrophoresis, Staining

Journal: Interdisciplinary Perspectives on Infectious Diseases

Article Title: The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

doi: 10.1155/2022/1639990

Figure Lengend Snippet: Percentage of apoptotic cells in mock- and NS1-transfected HEK-293T cells at 24 hr, 48 hr, and 72 hr posttransfection was determined by flow cytometry of cells that stained double-positive for PE Annexin V and 7-AAD.

Article Snippet: The full length of a synthetic gene encoding hPVB19 NS1 protein (NCBI Reference Sequence: NC_000883.2) was received in the pCMV6-AC-GFP-NS1 constitutive expression vector (Biomatik, Ontario, Canada).

Techniques: Transfection, Flow Cytometry, Staining

Journal: Interdisciplinary Perspectives on Infectious Diseases

Article Title: The Pathogenic Aspects of Human Parvovirus B19 NS1 Protein in Chronic and Inflammatory Diseases

doi: 10.1155/2022/1639990

Figure Lengend Snippet: Thirteen cytokines' concentration of mock-transfected, B19 NS1-transfected, and untransfected HEK-293T cell are measured at 24, 48, and 72 hr posttransfection. Cytokine concentrations are reported based on a picogram per milliliter as mean. The experiments were carried out two times in duplicate.

Article Snippet: The full length of a synthetic gene encoding hPVB19 NS1 protein (NCBI Reference Sequence: NC_000883.2) was received in the pCMV6-AC-GFP-NS1 constitutive expression vector (Biomatik, Ontario, Canada).

Techniques: Concentration Assay, Transfection